Expression data from human blastocyst stage embryo

Clinical data suggest that both undisturbed culture of embryos and selection algorithms based on TL morphometric parameters can in correlation and potentially improve embryo survival rates and live birth rates. However, there remains a need to validate and understand the mechanisms underlying the potential benefits of this technology remains. We used microarray to obtain and analyse transcriptome data of individual human embryos grown for up to 6 days to blastocyst in a TL incubator to study associations between morphokinetic TL parameters and the human embryo gene expression. Human embryos surplus to treatment requirements were donated with informed consent from several Assisted Reproduction Technology (ART) centres. Embryos were cultured to blastocyst in an EmbryoScope TL incubator and analysed by Affymetrix microarray.