Single cell sequencing of stromal vascular fraction (SVF) under B3-adrenergic agonist stimulation and mature adipocytes under cold exposure and B3-adrenergic agonist stimulation

A single cell suspension was generated from B3-adrenergic agonist (CL; 1 mg/kg/day) for 4 days and saline mouse SVF, and single-cell mRNAseq libraries were generated with Drop-Seq. A single cell suspension was generated from mouse mature adipocyte nuclei under the following conditions: RT, 24hrs @ 4 °C , 48hrs @ 4 °C, 4days @ 4 °C and B3-adrenergic agonist (CL; 1 mg/kg/day) and single-cell mRNAseq libraries were generated with 10X genomics. Overall design: Drop-Seq was performed on two separate days using the same age (10 week) and strain (C57BL/6) mice. 10X Genomics single cell sequencing was performed on three separate days using the same age (10 week) and two groups of C57BL/6 mice: C57BL/6 Il10raF/F and C57BL/6 AdIL10RaKO.