Endometriosis in mice and association with dysbiosis during the acute phase of lesion formation

The new formation of these lesions is associated with inflammation, angiogenesis and stem cell recruitment, which may lead to an altered composition of the gut mi-crobiota. To test this hypothesis, we herein induced endometriotic lesions by trans-plantation of uterine tissue fragments from green fluorescent protein (GFP)+ donor mice into the peritoneal cavity of GFP- C57BL/6 wild-type mice. Sham-transplanted animals served as controls. Fecal pellets of the animals were collected 3 days before transplantation as well as on day 7 and 21 after the induction of endometriosis to analyze the composition of the gut microbiota by means of 16S ribosomal-RNA gene sequencing.