The Gustatory Sensory G-Protein GNAT3 Suppresses Pancreatic Cancer Progression in Mice

Ablation of the gustatory G-protein, GNAT3, in damage and KRAS G12D induced pancreatic transformation enhanced CXCL1 and CXCL2 expression, altered the immunoregulatory gene expression of CXCR2 expressing myeloid-derived suppressor cells, and increased gMDSC presence to promote the progression of metastatic pancreatic ductal adenocarcinoma. Pancreata from Kras LSL-G12D/+; Ptf1a CreERT/+; R26 LSL-EYFP and GNAT3-ablated Kras LSL-G12D/+; Ptf1a CreERT/+; R26 LSL-EYFP mice were subject to single-cell RNA-seq using a droplet partitioning method (10X Genomics Chromium platform). Tissue from two mice per genotype were used in this study.