Data for: An injectable, degradable hydrogel plug for single-surgery tracheal occlusion in congenital diaphragmatic hernia (CDH)

Csv files containing raw data for rheological characterization of calcium alginate and methyl cellulose/hylauronan injectable hydrogels.

File name indicates sample type, concentration and test mode (time sweep, frequency sweep, stress sweep, stress ramp).

To prepare calcium alginate gels, alginic acid sodium salt (Fluka Analytical 71240, Mw = 1.2 - 1.9 x 105 g mol-1, M/G ratio = 1.56), was dissolved w/v in deionized water. CaCO3 (1/4 over alginate monomer moles) was added as the insoluble calcium source and vortexed until a homogeneous dispersion was obtained. Freshly made solution of D-Gluconic acid-δ-lactone (GDL) in deionized water was allowed to hydrolyse and reach a pH of 2.6 – 2.8 and mixed to Ca-alginate solutions at the same molarity of alginate and the mixtures was used after stirring for about 15 seconds.

Hyaluronan/methylcellulose (HA-MC) gels were prepared with MC with two different viscosities (HV = high viscosity MC - Methocel A4M and LV = low viscosity MC - A15 PREM LV) dissolved
in Dulbecco's phosphate-buffered saline (D-PBS) heated at 55 °C. After stirring, the solution was allowed to equilibrate at 4 °C overnight. Hyaluronic acid sodium salt from Streptococcus equi (Mw = 1.5 – 1.8 x 106 g mol-1) was added to MC solutions and again maintained overnight at 4 °C.

Rheological test were performed using a CVO 120 stress controlled Rotational Rheometer (Bohlin Instruments) using parallel plates, except for steady shear measurements where a cone-plate geometry (φ = 40 mm, θ = 1°) was used.

包含钙藻酸盐和甲基纤维素/透明质酸钠注射水凝胶流变学特性的原始数据CSV文件。文件名表明样品类型、浓度和测试模式（时间扫描、频率扫描、应力扫描、应力斜坡）。制备钙藻酸盐凝胶时，使用分析级海藻酸钠钠盐（Fluka Analytical 71240，分子量 = 1.2 - 1.9 x 10^5 g mol^-1，M/G比 = 1.56）以体积分数溶解于去离子水中。作为不溶性钙源，添加了CaCO3（超过藻酸盐单体摩尔数的1/4）并涡旋至获得均一分散。新鲜制备的D-葡萄糖酸-δ-内酯（GDL）去离子水溶液被允许水解并达到pH 2.6 – 2.8，与相同摩尔浓度的钙藻酸盐溶液混合，混合物搅拌约15秒后使用。制备透明质酸/甲基纤维素（HA-MC）凝胶时，使用两种不同粘度的甲基纤维素（HV = 高粘度甲基纤维素 - Methocel A4M 和 LV = 低粘度甲基纤维素 - A15 PREM LV），在55 °C加热的Dulbecco磷酸盐缓冲盐溶液（D-PBS）中溶解。搅拌后，溶液在4 °C下过夜平衡。添加Streptococcus equi来源的透明质酸钠盐（分子量 = 1.5 – 1.8 x 10^6 g mol^-1）到甲基纤维素溶液中，并在4 °C下过夜维持。流变学测试使用CVO 120应力控制旋转流变仪（Bohlin Instruments）进行，采用平行板，除了稳态剪切测量使用锥板几何形状（φ = 40 mm，θ = 1°）外。