RNA-seq analysis of NRF2 silenced human skin fibroblasts

This RNA-seq analysis was the beginning of our study, to have a first global unbiased approach to analyze the effect of NRF2 silencing on the mRNA profile of human skin fibroblasts. We demonstrated that besides NRF2 conventional antioxidant and metabolic target genes, tissue skeleton and especially matrisome genes were affected by NRF2 silencing. We further confirmed that NRF2 silencing some collagen genes, impacting collagen fibrillogenesis. We used primary papillary fibroblasts from 3 young donors, that we transfected either with a scramble siRNA or 3 different siRNA targeting NRF2, which is encoded by the NFE2L2 gene.