6A_Pvl phenotype quantification_I

Quantification of the protruding vulva (Pvl) phenotype of adult animals of indicated genotypes. Animals were grown at 20°C and examined at 52 h of development after plating synchronized L1 animals. N = 4 technical replicates. Condition Strain code Genotype wild type N2 wild type wild type* HW1792 lin-28(xe59[lin-28::flag::ha]) I lin-28(Δb)*; lin-46(++) HW3696 lin-28(xe344xe59[lin-28::flag::ha with exon1B(Δ)]) I; lin-46(xe370[5'UTR_GGAGmut]) V lin-28(Δb)*; let-7(++) HW3760 lin-28(xe344xe59[lin-28::flag::ha with exon1B(Δ)]) I; xeSi287[let-7p::let-7] V lin-28(Δb)* HW3381 lin-28(xe344xe59[lin-28::flag::ha with exon1B(Δ)]) I let-7(++) lin-46(++) HW3808 xeSi287[let-7p::let-7] lin-46(xe370[5'UTR_GGAGmut]) V lin-28(0) HW2705 lin-28(xe192) I lin-46(++) HW3531 lin-46(xe370[5'UTR_GGAGmut]) V let-7(++) HW1909 xeSi287[let-7p::let-7] V lin-28(Δb)*; let-7(++) lin-46(++) HW3810 lin-28(xe344xe59[lin-28::flag::ha with exon1B(Δ)]) I; xeSi287[let-7p::let-7] lin-46(xe370[5'UTR_GGAGmut]) V