Identification of stress response by multi-scale analyses of drought effect in Arabidopsis thaliana under future climate CO2

We used a multidisciplinary approach to investigate the interaction of drought and elevated CO2 in Arabidopsis thaliana leaves. Transcriptome and subsequent metabolite analyses identified a strong induction of the aliphatic glucosinolate (GL) biosynthesis as a main effect of eCO2 in drought-stressed leaves. We subjected Arabidopsis seedlings to a combination of two hydration (well-watered and drought) and CO2 levels (ambient (375±9 ppm; aCO2) and elevated (633±16 ppm; eCO2). To identify the molecular mechanisms involved in response to drought and eCO2, we performed a genome-wide transcriptome analysis on the developing rosette at 21 DAS, when it contains leaves in all stages of their development. Overall design: Twenty whole rosettes of Arabidposis plants were harvested at 21 days after sowing (DAS) from four treatments: 1) aCO2, 2) aCO2 + drought (40% Soil Water Content, SWC), 3) elevated CO2, 4) aCO2 + drought stress (40% SWC). Twenty whole rosettes per each biological replicate were collected. Three biological replicates used per each treatment (3 replicates x 4 conditions = 12 samples).