Alternative splicing pattern of SR protein genes in WT, HAP1/hap1-2, upf3-1, sr45-1, upf3-1;HAP1/hap1-2 (uh) and sr45-1;HAP1/hap1-2 (sh) mutant inflorescence tissues.

(A) Alternative splicing pattern was examined on RS40, RS41, RS31, SCL33, SR30, SCL30a, RS31a. GAPDH was used as control. Each band was corresponding to the each splicing isoform. The UTRs were presented by open boxes; the exons were presented by black boxes; the alternatively spliced exons were presented gray boxes, the introns were presented by lines. The position of gene-specific primers was shown by black arrowhead and the stop codons were shown as *. (B) The alternative splicing pattern of selective SR protein gene transcripts was confirmed by qPCR. The ratio of different splicing isoforms (AS) of each genotype was compared to WT (qrt) for student t-test (n = 3). * represents pp