Single-cell RNA sequencing on EM-E6/E7/TERT-1 endometrial epithelial cells

We report the single-cell high-throughput sequencing for non-tumorigenic EM-E6/E7/TERT-1(EME6/7t) endometrial epithelial cell line. EME6/7t cells were exposed to condition medium (CM) of adipose-derived stromal cells (ASCs) with or without tiplaxtinin (PAI-039). We employed unsupervised clustering analysis to characterize various PAI-1 related regulatory models in EME6/7t cells. This study provides a framework for exploring heterogeneous gene expression patterns caused by plasminogen activator inhibitor-1 (PAI-1) responses in the tumor microenvironment. Single-cell mRNA profiles of 1,911 EME6/7t cells were used to study and analyze PAI-1 responses in the development of endometrial cancer Please note that [1] the raw R1 reads was used to demultiplex row barcodes of column samples, and alignment was done on R2 reads. Therefore only R2 was provided and processed as single [2] the 'raw_to_processed_data_file.txt' contains the list of the raw data files associated with each processed data file.