miR-628-3p exerts a carcinogenic effect on hepatocellular carcinoma

Here, we evaluated the effect of miR-628-3p on HCC proliferation by in vitro proliferation assay and xenograft tumor model. Flow cytometry was used to monitor the cell cycle and apoptosis of HCC cells. Quantitative real-time polymerase chain reaction (qRT-PCR) and western blot (WB) were used to determine the expression of each gene.

本研究通过体外增殖试验和异种移植肿瘤模型，评估了miR-628-3p对肝细胞癌（HCC）增殖的影响。利用流式细胞术监测HCC细胞的细胞周期和凋亡情况，并通过定量实时聚合酶链反应（qRT-PCR）和蛋白质印迹法（WB）检测各基因的表达水平。