Identify molecules regulated by glucosylceramide synthase in mouse osteoblasts.

To identify molecules regulated by glucosylceramide synthase in osteoblasts, we conducted microarray analysis using samles treated with the inhibitors of glucosylceramide synthase. Four groups for MC3T3-E1 cells were Control, Miglustat, D-PDMP, and D-PPMP. The concentrations of Miglustat, D-PDMP, and D-PPMP were 50 microM, 10 microM, and 1.0 microM, respectively. Four days after treatment of their inhibitors of GCS, the cells were plated at 2.5 x 100000 cells into 60 mm dishes, and cultured for 24 h. The cells were harvested for microarray analysis.