Gene expression in murine embryonic fibroblasts stimulated with DNA or LPS

MyD88 may play a direct role in STING-dependent signaling, or alternatively that STING-dependent pro-inflammatory cytokines may require downstream MyD88-dependent signaling to exert their effect. To determine this, we treated STING or MyD88-deficient murine embryonic fibroblasts (MEFs), bone marrow derived macrophages (BMDM) or dendritic cells (BMDC) with exogenous CDN’s or cytosolic dsDNA (ISD) which triggers STING-signaling and type I IFN production. To evalulate the gene expression profiling, we isolated mouse embryonic fibroblast from WT, SKO, or MyD99KO mice and treated with dsDNA or LPS for 6 hours.