Sequencing of capped and uncapped RNAs in the antennae of H. saltator

5' phosphorylated RNAs were captured by direct adapter ligation to phosphorylated 5' ends, as in the degradome method. To capture 5' capped RNAs, total RNA was first dephosphorylated to remove 5' phosphorylated species, and then decapped, which resulted in phosphorylated 5' ends of formerly capped molecules only. This approach is based on the TERA-Seq method.