小鼠躯体感觉系统单细胞投射谱

躯体感觉系统处理多种模态的躯体感觉信息，是机体发现潜在危险和维持生存的关键节点。我们结合了稀疏标记、fMOST成像和单神经元追踪等技术，绘制了785个小鼠颈段脊髓投射神经元和1463个接收脊髓输入的脑内中继神经元的单细胞投射谱，这些中继神经元分布于丘脑、臂旁核、上丘运动区、下丘脑、导水管周围灰质和延髓，我们鉴定出了19个脊髓投射神经元亚型和34个中继神经元亚型。我们绘制了躯体感觉信息上行通路的连接网络图，通过对该网络的深入分析，我们系统地解析了脊髓上行通路的解剖结构，揭示了躯体感觉系统的组织规律，为后续躯体感觉信息处理的神经环路机制研究奠定了坚实的基础。

The somatosensory system processes multimodal somatosensory information and acts as a critical hub for organisms to detect potential dangers and sustain survival. We integrated techniques including sparse labeling, fMOST imaging, and single-neuron tracing to map the single-neuron projection profiles of 785 cervical spinal cord projection neurons and 1463 intracerebral relay neurons receiving spinal inputs in mice. These relay neurons are distributed across the thalamus, parabrachial nucleus, motor region of the superior colliculus, hypothalamus, periaqueductal gray, and medulla oblongata. We identified 19 subtypes of spinal cord projection neurons and 34 subtypes of relay neurons. We constructed a connectivity network map of the ascending somatosensory information pathway. Through in-depth analysis of this network, we systematically deciphered the anatomical architecture of the spinal ascending pathway, revealed the organizational principles of the somatosensory system, and laid a solid foundation for subsequent studies on the neural circuit mechanisms of somatosensory information processing.

搜集汇总

背景与挑战

背景概述

该数据集通过稀疏标记和fMOST技术，绘制了785个小鼠脊髓投射神经元和1463个中枢中继神经元的单细胞投射图谱，并鉴定了19个脊髓投射神经元亚型和34个中枢中继神经元亚型，构建了躯体感觉上行通路的全面连接图。