Transcriptional profiling of human pancreatic tumor cells under nutrient and oxygen deprivation condition, with and without CRISPR targeting of BCL2L1 gene

Transcriptional profiling showed that adaptation to nutrient and oxygen deprivation condition is associated with widespread transcriptional reprogramming resulting in the induction of glycolysis and autophagy and repression of the TCA cycle and biosynthesis Human pancreatic tumor cells transduced with control or CRISPR targeting BCL2L1 were cultured in nutrient and oxygen deprivation medium for 2 days and then were collected for RNAseq.