Segregation Patterns and RAD Alleles for P. xylostella linkage groups.

Maternally derived segregation patterns (for RAD alleles present in the mother but absent in the father) for the 62 chromosomes of P. xylostella fall into 31 pairs. Offspring segregation patterns are presented as binary strings, where 1 indicates presence of RAD allele and 0 indicates absence. The two sets of digits separated by a space are the Control and Bioassay individuals (Control 5 and Bioassay 12 are not included). For example, Segregation Pattern 1 for PxLG5 (the resistance chromosome) is present in Controls 1, 8 and 9 and absent in all other individuals, including all Bioassay survivors. 108 candidate RAD alleles had this segregation pattern, with 82 candidate RAD alleles having the inverse pattern. The segregation patterns for PxLG1 identify the sex chromosomes (sexes segregate FMFMMMFFFMMMMMFFFMMM; see Table 1). Segregation Pattern 2 is the W chromosome (present in females, absent in males) and Segregation Pattern 1 the Z chromosome.