Cesarean section-induced changes in the gut microbiota facilitate metabolic disease in high-fat diet-induced obese mice

Growing evidence suggests that CS birth may alter the gut microbiota (i.e. dysbiosis), increasing the risk of immune and metabolic disorders. This study aims to investigate the causal relationship between caesarean section (CS)-induced gut dysbiosis and obesity in a high-fat diet mouse model. In this study, sera samples from male C57BL/6 mice were delivered via CS or vaginally (VD) at the age of 4 weeks were profiled for metabolomics by LC-MS. After preprocessing with XCMS, MS features showing significant difference in peak height between the two groups were annotated. The CS microbiome associated mice had higher levels of indoleactic acid, isovalerylcarnitine and pantothenic acid, the VD microbiome associated mice had higher levels of cholic acid and lysophosphatidylcholine 16:0.