HopZ1a promotes GmJAZ1 degradation in planta.
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https://figshare.com/articles/dataset/_HopZ1a_promotes_GmJAZ1_degradation_in_planta_/839617
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(A) HopZ1a induces the degradation of GmJAZ1 when the proteins were co-expressed in N. benthamiana. GmJAZ1-FLAG and HopZ1a-HA were transiently expressed in N. benthamiana and the abundance of GmJAZ1 was detected by western blots at 20 hpi. The same protein gel was stained with Coomassie blue to show equal loading. (B) HopZ1a induces GmJAZ1 degradation using a semi-in vitro assay. GmJAZ1-FLAG and 3×FLAG-HopZ1a were transiently expressed in N. benthamiana individually. Total proteins were extracted from the infiltrated leaves 20 hours post Agro-infiltration, mixed in equal volume, and incubated at 4°C for six hours. The abundance of GmJAZ1-FLAG was then analyzed by western blots. These experiments were repeated three times with similar results.
创建时间：
2013-10-31
