Cell size and cell number changes during <i>Nematostella</i> growth and shrinkage (Fig. 3)

Individual polyps under ad libitum feeding (0d-2d-4d-6d-10d) and starvation conditions (1 day post feeding, 2dpf, 5dpf, 12dpf, 21dpf as well as re-fed samples (20d starved+24h post re-feeding)) were imaged for body size measurement and then dissociated using ACME. Cells were stained with 1μg/ml FxCycle violet DNA dye (Invitrogen) and red fluorescent beads (F8834, Invitrogen) were added to infer the total number of cells per individual. In the starvation dataset a known number of yellow-green fluorescent beads (F8836, Invitrogen) was added before ACME to estimate and compensate for cell loss during dissociation.Flow cytometry was performed a BD LSRFortessa (BD Life Sciences) and the resulting data was analysed using FlowJoV10.8 (BD Life Sciences)..wsp files contain the samples, gating strategy and cell populations used for the analysis in FlowJo.fcs files represent the individual flow cytometry output files that were analysed in the .wsp file<br>

对自由摄食（0d、2d、4d、6d、10d）及饥饿条件下的单个水螅体（其中饥饿条件涵盖摄食后1天、受精后2天、5天、12天、21天，以及复喂样本：饥饿20天+复喂24小时）进行成像以测量体型，随后使用ACME进行细胞解离。采用1μg/mL的FxCycle Violet DNA染料（Invitrogen）对解离所得细胞进行染色，并加入红色荧光微球（F8834，Invitrogen）以推算每个个体的总细胞数。在饥饿组数据集的ACME解离步骤前，加入已知数量的黄绿色荧光微球（F8836，Invitrogen），用于估算并补偿解离过程中发生的细胞损失。采用BD LSRFortessa流式细胞仪（BD Life Sciences）完成流式细胞术（Flow Cytometry）检测，所得数据通过FlowJoV10.8软件（BD Life Sciences）进行分析。.wsp文件包含了FlowJo分析所需的样本、设门策略及细胞群信息；fcs文件则为该.wsp文件中所分析的单一样本流式细胞术输出文件。