RNAseq of CD4 T cells treated with survivivin inhibitor YM155 for 24h

CD4 T cells from 4 healthy women were activated with aCD3 and treated with the survivin inhibitor YM155, 10 ng/ml or control during 24 hours. The last 2 hours with upplement of IFNg. The objective was to understand the action of survivin (coded by BIRC5 gene) in CD4 T cells. Overall design: 4 healthy women, paired analysis possible. CD4 cells from human PBMC isolated by positive selection on Dynabeads (cat.11331D), stimulated with anti-CD3 (OKT3, Sigma-Aldrich) treated for 24h with YM155, 0 or 10nM, (Selleck Chemicals, Houston, TX) last 2 hours with addition of IFNg (50ng/ml, Peprotech, Cranbury, NJ, USA)