Clock rescue in the brain restores 12- and 24- hour rhythms in the liver

Circadian profiling was performed in mouse livers from three different genotypes/treatments: Wildtype, Clock mutant, and brain-specific Clock rescue. Rescuing Clock specifically in the brain partially rescued robust circadian and harmonic rhythms. Samples were collected every 2 hours for 48 hours from 2-4 mice per time point; samples were pooled and analyzed using Affymetrix Mouse Exon arrays, analyzed at the gene level.本研究针对三种不同基因型/处理方式的小鼠肝脏开展昼夜节律谱分析：野生型（Wildtype）、Clock突变型（Clock mutant）以及脑特异性Clock挽救处理（brain-specific Clock rescue）。在大脑中特异性挽救Clock基因，可部分恢复稳健的昼夜节律与谐波节律。实验每2小时采集一次样本，持续时长共计48小时，每个时间点取2-4只小鼠的样本；随后将各时间点的样本混合，使用Affymetrix小鼠外显子芯片（Affymetrix Mouse Exon arrays）进行检测，并在基因层面完成数据分析。