Whole cell patch-clamp recordings of cerebellar Golgi cells

The Golgi cells, together with granule cells and mossy fibers, form a neuronal microcircuit regulating information transfer at the cerebellum input stage. In order to further investigate the Golgi cells properties and their excitatory synapses, whole-cell patch-clamp recordings were performed on acute parasagittal cerebellar slices obtained from juvenile GlyT2-GFP mice (p16-p21). Passive Golgi cells parameters were extracted in voltage-clamp mode by analyzing current relaxation induced by step voltage changes (IV protocol). Excitatory synaptic transmission properties were investigated by electrical stimulation of the mossy fibers bundle (5 pulses at 50 Hz, EPSC protocol, voltage-clamp mode.