青藏高原草地土壤古菌数据集（2020-2021）
收藏资源简介：
本数据集包含青藏高原草地土壤理化性质数据，包括样品编号、采集人、采集时间、采集地点、经纬度、海拔、植被类型、取样深度等信息，其中样品编号对应于古菌和原生生物双端测序结果编号。数据来源于第二次青藏高原科学考察项目，于2021年对青藏高原草地土壤进行调查采样，通过室内化验分析获得的一手数据，数据质量通过测定空白样品、重复样品和标准样品进行统一控制。数据包含基本样点信息和土壤微生物古菌测序数据。使用引物组Arch519F（CAGCCGCCGCGGTAA）/Arch915R（GTGCTCCCCCGCCAATTCCT）扩增古菌16S rRNA基因。使用QIAquick纯化试剂盒（Qiagen）纯化PCR产物，并按等摩尔比混合。使用NovaSeq 6000平台（美国Illumina, San Diego）和Majorbio Bio-Pharm Technology（中国上海Majorbio）的流动池进行扩增子的双端测序。该数据集可用于气候变化和人类活动影响下土壤质量和功能以及物种多样性多样性分布特征等研究。
This dataset contains physicochemical property data of grassland soils on the Tibetan Plateau, including information such as sample ID, collector, collection date, collection location, latitude and longitude, elevation, vegetation type, and sampling depth. The sample ID corresponds to the paired-end sequencing result IDs for archaea and protists. This dataset is derived from the Second Tibetan Plateau Scientific Expedition and Research (STEP) Program. Survey and sampling of grassland soils on the Tibetan Plateau was conducted in 2021, and raw data were obtained via laboratory assays. The data quality was uniformly controlled by assaying blank samples, replicate samples and standard reference samples. The dataset includes basic sample site information and soil microbial archaeal sequencing data. The archaeal 16S rRNA gene was amplified using the primer pair Arch519F (CAGCCGCCGCGGTAA)/Arch915R (GTGCTCCCCCGCCAATTCCT). PCR products were purified using the QIAquick Purification Kit (Qiagen) and pooled at equimolar concentrations. Paired-end sequencing of the amplicons was performed on the NovaSeq 6000 platform (Illumina, San Diego, USA) using flow cells from Majorbio Bio-Pharm Technology (Majorbio, Shanghai, China). This dataset can be used for studies on soil quality and function, as well as the distribution characteristics of species diversity under the impacts of climate change and human activities.
