Analysis of genetic variability in DENV-1 at different levels of evolutionary divergence based on a 758 nt fragment in the E-gene.

aSequences in datasets ��Serotype 1��, ��Genotype IV�� and ��FP 2001�C2006�� were also used for phylogenetic reconstructions (Figures 2, 3, S1, S2).bThe average of the results obtained for each individual (Table 5) was used for intra-host data analysis.The average pairwise distance was calculated among the nucleotide sequences in each dataset (�� nt).The mean ratio of synonymous (dS) and non synonymous mutations per site (dN) were estimated using the pairwise method of Nei and Gojobori.ABBREVIATIONSaa: amino acidDENV: dengue virusDF: dengue feverDHF: dengue haemorrhagic feverDSS: dengue shock syndromedN: ratio of nonsynonymous substitutions per sitedS: ratio of synonymous substitutions per siteFP: French Polynesiant:nucleotideRT-PCR: reverse transcription-polymerase chain reaction�� nt: nucleotide sequence divergence (pairwise distance)