Data for the article entitled: Efficient multiphoton microscopy with picosecond laser pulses

The following files include data presented in the manuscript “Efficient multiphoton microscopy with picosecond laser pulses,” written by Katarzyna Kunio, Jakub Bogusławski, and Grzegorz Soboń.Figure 2a – optical spectrum of the picosecond oscillator measured directly at the oscillator output and its spectral phaseFigure 2b – output pulse autocorrelation measured directly at the oscillator output together with Gaussian fit functionFigure 3a_1 – autocorrelation of the picosecond pulse with a Gaussian fit measured at the laser system output (after AOM)Figure 3a_2 – autocorrelation of the picosecond pulse with a Gaussian fit measured at the the microscope’s sample planeFigure 3b – comparison of the reconstructed temporal profile of the femtosecond pulse at the laser system output (after AOM) and in the microscope’s sample plane.Figure 4a – TPE fluorescence images of convallaria majalis sample obtained using femtosecond pulse train (scale bar: 75 µm): 227 fs, 15 MHz, 0.7 mW.Figure 4b – TPE fluorescence images of convallaria majalis sample obtained using picosecond pulse train (scale bar: 75 µm): 10 ps, 0.3 MHz, 0.7 mW.Figure 4c – SHG images of urea microcrystals using femtosecond pulse train (scale bar: 200 µm): 227 fs, 15 MHz, 0.3 mW.Figure 4d – SHG images of urea microcrystals using picosecond pulse train (scale bar: 200 µm): 10 ps, 0.3 MHz, 0.3 mW.Figure 4e – pixel intensity histograms of images shown in Fig. 4a and b (convallaria majalis sample, TPE fluorescence).Figure 4f – pixel intensity histograms of images shown in Fig. 4c and d (urea microcrystals, SHG).Figure 5a – TPE fluorescence images of convallaria majalis sample obtained using femtosecond pulse train (scale bar: 75 µm): 227 fs, 15 MHz, 0.7 mW.Figure 5b – TPE fluorescence images of convallaria majalis sample obtained using picosecond pulse train (scale bar: 75 µm): 10 ps, 1.1 MHz, 0.7 mW.Figure 5c – SHG images of urea microcrystals using femtosecond pulse train (scale bar: 200 µm): 227 fs, 15 MHz, 0.3 mW.Figure 5d – SHG images of urea microcrystals using picosecond pulse train (scale bar: 200 µm): 10 ps, 1.1 MHz, 0.3 mW.Figure 5e – pixel intensity histograms of images shown in Fig. 5a and b (convallaria majalis sample, TPE fluorescence).Figure 5f – pixel intensity histograms of images shown in Fig. 5c and d (urea microcrystals, SHG).