Mus musculus Transcriptome or Gene expression. Mus musculus

This study aims to examine changes to the microRNA biogenesis pathway when the exonuclease Dicer is mutated such that it cannot recruit its RNA-binding partner proteins, TRBP and PACT. These partner proteins may contribute to changes in the processing of microRNAs, including effects on microRNA length and their loading onto targeting effector protein Ago2. The study used immunoprecipitation of Ago2 followed by sequencing of associated microRNAs to text for differences between cells containing Dicer that could recruit TRBP/PACT and those that could not