Expression data from spermatogonia enriched from 12 dpc mouse testes

Spermatogonial stem cells (SSCs) cotinues to produce sperm throughout life by self-renewal division. Although it is possible to expand SSCs from most of the mouse strains in vitro for a long-term period by supplementing GDNF and FGF2, SSCs from a 129 background do not proliferate under the same culture conditions. Since it suggested that they have distinct self-renewal requirements, gene expressions of spermatogonia enriched from testes of DBA/2, C57BL/6, and 129 strains were compared. In this dataset, we include the expression data obtained from spermatogonia enriched from 12dpc testes of DBA/2, C57BL/6, and 129. Enrichment was performed by magnetic activated-cell sorting using anti-Ecadherin (CDH1) antibody (ECCD2). These data are used to obtain 359 genes that are differentially expressed among DBA/2, C57BL/6, and 129. 3 Total samples were analyzed. We generated the following comparisons using GeneSpring: 129B6; 129DBA/2. Genes with an FDR≤20% and a fold-change ≥2 were selected.