Additional file 17: of Comprehensive assessment of multiple biases in small RNA sequencing reveals significant differences in the performance of widely used methods

Table S13. Evaluation of the most abundant miRNA in the NEXTflex samples. To determine if our UMI length was adequate, we estimated the number of starting sequences for the most abundant miRNA (miR-9-5p) in the NEXTflex data. For comparison sake, we also show the estimated number of starting molecules for a more moderately abundant miRNA (miR-137). The possible number of starting sequences for each miRNA was estimated assuming a PCR amplification efficiency of only 62.5%, all samples, thus enabling more conservative estimates for the number of starting sequences. PCR efficiencies of miRNA generally range from 75 to 95%, and previous studies demonstrate that the PCR efficiency of miR-9-5p has been 98%. Therefore, our estimates of the number of starting miR-9-5p sequences are likely higher than the true number of starting sequences. (XLSX 44 kb)