Identifying a Novel Connection Between the Fungal Plasma Membrane and pH Sensing

To identify cellular processes similarly regulated by both Rim101 and Cdc50, we compared the transcriptomes of the rim101∆ and cdc50∆ strains against WT. We observed a partial overlap in the transcriptional perturbation shared by the mutant strains. Most notably, these data demonstrate a role for both Rim101 and Cdc50 in regulating the gene expression of membrane-associated proteins, including those involved in lipid bilayer composition and organization. Furthermore, this class of membrane-associated genes co-regulated by Rim101 and Cdc50 demonstrate similar expression changes in the two mutant strains in both direction and magnitude. Gene expression analysis of WT and rim101∆ and cdc50∆ mutant cells generated in triplicate by RNA-Sequencing.