Macrophage-glia interactions regulate immune-damage to enteric neurons during West Nile virus infection [Dataset]

In this study, we defined the neuron-glia-macrophage interactions after West Nile virus (WNV) infection; this model neurotropic virus causes GI tract dysmotility in mice via injury of enteric neurons through a T cell-mediated cytolytic mechanism. Our experiments demonstrate that resident macrophages play a critical role in regulating the glial immune response to viral infection in the intestinal muscularis, and that their depletion markedly exacerbates neuronal injury. Data were generated using flow cytometry, fluorescent activated cell sorting, qRT-PCR, immunofluorescent confocal microscopy, RNA sequencing, fluorescent in situ hybridization. There are 4 main Figures and 4 Supplementary Figures. Data were obtained using C57BL6/J mice and Ai3 (YFP) (B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J, Cx3cr1CreERT2 (B6.129P2(C)-Cx3cr1tm2.1(cre/ERT)Jung/J), Plp1CreERT2 B6.Cg-Tg(Plp1-cre/ERT)3Pop/J); NuTRAP (B6;129S6-Gt(ROSA)26Sortm2(CAG-NuTRAP)Evdr/J) mice; DTA (B6.129P2-Gt(ROSA)26Sortm1(DTA)Lky/J mice.