Bulk RNA-Seq of whole testis sampled from juvenile mice

Spermatogenesis is a recurring differentiation process that results in the production of male gametes within the testes. During this process, spermatogonial stem cells differentiate to form spermatocytes, which undergo two rounds of meiotic division to form haploid spermatids. Throughout spermiogenesis, round spermatids elongate to form mature sperm. To profile maturing cell types, we generated bulk RNA-seq data from whole testis undergoing the first round of spermatogenesis between post-natal day P6 and P35. We also compared these libraries to adult samples.精子发生（Spermatogenesis）是一种周期性发生的细胞分化过程，可在睾丸内产生雄性配子。在此过程中，精原干细胞（spermatogonial stem cells）先分化为精母细胞（spermatocytes），精母细胞经两轮减数分裂后形成单倍体精子细胞（haploid spermatids）；在精子形成（spermiogenesis）阶段，圆形精子细胞会伸长并最终形成成熟精子。为了对成熟过程中的各类细胞进行转录组分型，我们对出生后第6天（P6）至第35天（P35）期间处于第一轮精子发生过程的完整睾丸组织开展了批量RNA测序（bulk RNA-seq），获取了其转录组数据。同时，我们还将该批次测序文库与成年睾丸样本进行了对照分析。