Raw data experiments Lopez-Arias et al 2017.xlsx

We sistematically compared the effect of neuronal expression of secretable forms of the <b>amyloid peptides</b> <b>Abeta40, Abeta42 or Abeta42arc</b>, with normal or elevated PI3K levels, on synaptic parameters of the <b><i>Drosophila</i></b> adult neuromuscular junction (NMJ), on brain neurodegeneration, and on longevity.<br><br>For SYNAPTIC PARAMETERS, the number of <b>BRANCHES</b>, <b>BOUTONS</b>, and <b>SYNAPSES</b> (Bruchpilot-positive active zones) was quantified in the adult abdominal ventral NMJ at different <b>AGE</b>s (days after eclosion). <br><br><b>WORKSHEET "SEGMENT AND AGE"</b>: data from wild type males of different ages (from 3 to 45 days after eclosion) in different <b>SEGMENTS</b> (A3, A4 and A5).<br><br><b>WORKSHEETS "NMJ+Abeta"</b>: data from A3 ventral NMJs of control males and males expressing each Abeta peptide in motor neurons, at 5 ages (3, 7, 15, 20 and 30 days after eclosion; at 30 days after eclosion, most Abeta42arc flies had dies, and therefore there is no data for this genotype/age). The box contains the key for the <b>GENOTYPE</b>s used. <b>FLY#</b> identifies each male with a unique number (with data from either the rigth or the left, or both, NMJs). <br><br><b>WORKSHEETS "NMJ+Abeta+PI3K"</b>: data from A3 ventral NMJs of control males and males co-expressing elevated PI3K levels and each Abeta peptide in motor neurons, at 2 ages (15 and 20 days after eclosion). The box contains the key for the <b>GENOTYPE</b>s used. <b>FLY#</b> identifies each male with a unique number (with data from either the rigth or the left, or both, NMJs).<br><br><b>WORKSHEET "NEURODEGENERATION":</b> the percentage of neurodegenerated area (<b>%NEURODEGENERATION</b>) was measured at 15 and 20 days after eclosion (<b>AGE</b>) in whole brains from control males and males with pan-neural expression of each Abeta peptide and normal or elevated PI3K levels. The box contains the key for the <b>GENOTYPE</b>s used.<br><br><b>WORKSHEET "LONGEVITY OASIS"</b>: survival data from control males and males expressing each Abeta peptide with normal or elevated PI3K levels. Data are formatted for analysis with the online analysis survival package OASIS (doi:10.1371/journal.pone.0023525).The box contains the key for the <b>GENOTYPE</b>s used. For each genotype (<b>%N</b>), each line depicts the number of "<b>dead</b>" and "<b>censored</b>" males at a given age (<b>#days</b>).