Cardiac Outflow tract septation defects in a DiGeorge syndrome model respond to Minoxidil treatment

We found that Tbx1 suppresses a subset of ECM-related genes in a cell differentiation model. Treatment with a Lysyl hydroxylase inhibitor drug in vivo during pregnancy reduced the expressivity of cardiac defects in Tbx1 mutant embryos. E14-Tg2a cells (parental line and Tbx1-/- line 4D) were dissociated with Trypsin-EDTA and cultured at a density of 100,000 cells/ml in serum-free differentiation media. We followed the protocol described by Andersen et al Nat Commun 9, 3140 (2018) and harvested cells at day 6 of differentiation. We processed for QuantSeq 4 biological replicates of the parental line and 4 of the 4D line.