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Quantification of T Cell Polarization.

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https://figshare.com/articles/dataset/_Quantification_of_T_Cell_Polarization_/493383
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资源简介:
Primary CD4+ T cells and P2 cells infected with VSV-G-pseudotyped HIV-1 encoding Gag-YFP were cultured for two days, fixed, immunostained for cellular proteins, and examined by fluorescence microscopy. Gag-YFP-positive and -negative cells were categorized based on cell morphology, and cells in each category were counted. Cells with circularity below 0.8 (see Materials and Methods) were categorized as polarized cells.
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2010-10-28
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