Surface soil collected from a farm in Waseca County, Minnesota.. soil metagenome

Surface soil (0-10 cm) was collected in September 2001 from a farm in Waseca County, Minnesota. Biochemical analysis on 20 g of soil from the same site revealed it to be clay loam, with fair to low organic matter content, high levels of essential elements, and low levels of nonessential elements. Microscopic analysis, including SYBR green DNA staining, found the organisms in the sample to be primarily prokaryotic. PCR-amplified 16S rRNA sequences from the sample revealed the presence of numerous bacterial and archaeal lineages. The WGS project and sequences deposited into the Trace Archive can be found using the Project data link.