Differentially expressed lncRNA&mRNA between macrophage M0 and macrophage M2 cells

To compare lncRNA&mRNA expression diffrence between macrophage M0 and macrophage M2 cells, we performed microarray using Arraystar Human LncRNA + mRNA Microarray v3.0 (8×60K) platform Total RNAs from six independent groups of M0 macrophages or M2 macrophages were isolated using Trizol reagent (Invitrogen life technologies, CA, USA),and labeled using Quick Amp Labeling Kit, One-Color (Agilent p/n 5190-0442). Next, the labeled/amplified RNA and labeled cRNA was purified and hybridizated using Agilent Gene Expression Hybridization Kit (Agilent p/n 5188-5242). Microarray slides were washed and scanned on an Agilent Microarray Scanner (Agilent p/n G2565BA), and data were extracted using Agilent Feature Extraction Software.