The transcriptomic differences between Th1, Tr1, and Tneg cells in controlled human malaria infection. The transcriptomic differences between Th1, Tr1, and Tneg cells in controlled human malaria infection

This study was designed to determine the differences in gene transcripts between CD4+ T helper (Th)1, Tr1, and Tneg cells during controlled human malaria infection. Overall design: Bulk RNA-sequencing was performed on CD4+ T helper (Th)1 (IFNγ+ IL-10-), Tr1 (IFNγ+ IL-10+), and Tneg (IFNγ- IL-10-) cells sorted from the peripheral blood mononuclear cells (PBMCs) of n = 6 participants in a controlled human malaria infection trial at day 14 p.i. Cells were stimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin prior to sorting by cytokine capture flow cytometry.