Genes required for serum resistance identified by multiplexed TraDIS.

aSerum sensitivity was shown as −log10 of viable count difference between t = 90 min and t = 0 min. The higher the number the more sensitive the mutant is.bSerum sensitivity was determined by competitive assays: 0 means no difference was found between the wild-type and mutant; 1 means the mutant was more attenuated than the wild-type.cLPS patterns were coded as followed: WT, similar to wild-type strain, no change observed; LA, only the band for lipid A-core observed; LA+1, lipid A-core band and only one band above it; X, changes observed, see Supplementary Figure S3 for band patterns.dThe wild-type (WT) MIC is 0.8 M for NaCl and 0.125% for SDS.