Transcriptomic analysis of Retinoic acid and 9 cis retinoic acid in zebrafish.. Danio rerio

We are presenting the application of toxicogenomics in the evaluation of the toxic effects of retinoic acid and one of its isoforms the 9-cis retinoic acid. The main goal is to distinguish the pattern of action of the both chemical compounds and their action in an extended exposure. The results suggest a different pattern within the days and the chemicals. Representatives of each GO functional groups were selected and quantified by real-time PCR to validate the microarray data and to differentiate the action of retinoic acid compounds studied. Overall design: Two different treatments were performed in this study. Zebrafish fertlized embryos were exposed to retinoic acid and 9-cis retinoic acid, during 24h and 72h. 3 biological replicates were performed per treatment.