Chronic low dose-rate versus acute ionizing radiation induced gene expression in primary derived prostate fibroblasts. Homo sapiens

Using a novel flood source construct, we performed a direct comparison of genome-wide gene expression regulations resulting from exposure of primary human prostate fibroblast cultures to acute (10cGy and 200cGy) and longer-term chronic (1.0-2.45cGy cumulative over 24hr) exposures. Overall design: Samples were collected at 24 hours after an acute 10cGy or 200cGy exposure (48cGy per minute) versus immediately after an accumulated 24 hour chronic 1.0 to 2.45cGy low dose-rate exposure (7 to 17µGy per minute). Control 0cGy samples were collected at the same time. A total of 4 independent experimental replicates were used for each sample resulting in (2 individuals × 4 experimental conditions × 4 experimental replicates) 32 microarrays.