Glutathione triggers leaf-to-leaf, calcium-based plant defense signaling

To get deeper insights into the GSH-triggered systemic defense signaling events in plants, we performed transcriptome profiling on leaf 6 collected after one hour application of GSH to leaf 1 of WT plants. To further investigate the relations between GSH and Glu in plant wound response, we generated transcriptome data sets from leaf 6 one hour after wounding leaf 1 or application of Glu to leaf 1 of WT plants. Arabidopsis WT plants growing in MS medium at 22℃ with a 10 h/14 h light/dark photoperiod for 22-24 days were used for GSH, Glu, and wounding treatment. Transcriptome profiling was performed on leaf 6 respectively collected one hour after wounding leaf 1 or application of 100 mM GSH or 100 mM Glu to leaf 1 of WT plants.