Dataset on the Influence of Crosslinker Length on Antibody Orientation and the Performance of DLS-Based SARS-CoV-2 Biosensors

Figure DB1. Characterization of the synthesized AuNPs revealed unimodal size distributions and red shifts in the plasmon resonance corresponding to progressive particle growth, confirming the successful synthesis of monodisperse, high-quality gold nanoparticles
Figure DB2. SEM images of the AuNP sample (D53) showing a Feret diameter of 56.2 ± 6.1 nm, spherical morphology, and a unimodal diameter distribution.
Figure DB3. ATR-FTIR spectra of the functionalized AuNPs showing characteristic bands of alkyl (C–H) chains at 2926 and 2848 cm⁻¹, and functional groups associated with carboxylic acids, including carbonyl (C=O) at 1647–1645 cm⁻¹ and carboxylate (COO⁻) at 1559–1556 cm⁻¹.
Figure DB4. Details in FTIR spectra of AuNPs@crosslinkers: (a) CH2 stretching modes and (b) CH2 asymmetric band position as a function of the crosslinker’s chain size, in number of carbon atoms.
Figure DB5. Deconvolution of the amide I bands for AuNPs@crosslinker-pAb revealed changes in the secondary structure profiles.
Figure DB6. Stability analysis of the biosensor based on variations in hydrodynamic diameter and polydispersity index over a 60-day period.
Figure DB7. BCA assay results for quantification of unbound polyclonal antibodies after conjugation, showing that AuNPs@MUA-pAb consistently exhibited the lowest concentration of unbound antibodies.