Transgene chromosomal insertion sites.

The table shows type of transgene cassette (Gal4 or UAS); the name of the line; the chromosomal integration site (Chr) into the genome (if known) by chromosome name and band (UN indicates that the region is located on an unknown chromosome); the proximity to nearest predicted gene in VectorBase, the D. melanogaster orthologue in intragenic insertions, and the contig number of unknown chromosome insertion sites (location); the sequence obtained by inverse PCR in both directions from the insertion site (sequenced region); and GenBank accession numbers of flanking sequences longer than the submission threshold of 200 bp (GenBank). Of the five insertions into annotated genes, four (Mvs, Nbt, Wby and Xnt) are 1∶1 orthologues of D. melanogaster genes and one (Mbl) is part of an orthologous group as predicted in VectorBase.*Line Wlm also displays salivary gland CFP expression. It also contains two transposons, one canonical.**and one containing piggyBac sequences indicative of a non-TTAA dependant insertion (not shown).