Modified strain with alterations to the chromsome. Mycoplasma mycoides subsp. capri str. GM12

M. mycoides subspecies capri str. GM12 clone deltatypeIIIres. The GM12 strain was originally isolated by Al DaMassa. The J. Craig Venter Institute obtained the strain from Dr. Mary Brown at the University of Florida. In 2009 the organism was renamed. The J. Craig Venter Institute (JCVI) used it as a donor genome in the first demonstration of bacterial genome transplantation. In 2009 the JCVI cloned the genome into Saccharomyces cerevisiae as a yeast centromeric plasmid. The JCVI then used yeast genetic tools to delete part of a type III restriction enzyme, a modification that could not be made using existing genetic tools for M. mycoides. Then the genome was transplanted from yeast into a Mycoplasma capricolum recipient cell to generate a novel mutant M. mycoides subspecies capri. This is the first report of transplanting a genome from a prokaryote, to a eukaryote, and then back to a prokaryote