Global changes in granulosa cell transcription in response to the LH surge

During ovulation, the LH surge leads to the activation of various signalling cascades in granulosa cells, resulting in a critical shift in chromatin landscape and correspondingly the ovarian gene expression profile. Such large-scale genomic reprogramming involves a specific suite of ovulatory transcription factors. This study aims to characterise global changes in the chromatin landscape that are induced by the LH surge in granulosa cells during ovulation. RNA-seq was performed on 4 replicates of granulosa cells obtained from eCG-stimulated mice after 0 h or 8 h post-hCG treatment