Identification of uterine regeneration promoting molecuar signaling pathways in a mouse model of decellularized matrix transplantation.. Mus musculus

Recently, we have reported the successful reconstruction of functional mouse uteri using a technique of decellularized matrix transplantaion, in which decellularized uterine matrices treated with SDS from donor mouse uteri were transplanted into the artificial defects of recipient mouse uteri, and discovered that STAT3 accelerates uterine regeneration. In this project, we aims to identify more specific molecular regulation mechanism downstream of STAT3, comparing transcriptome among regenerating epithelium, cell-supplying epithelium in the surgical sites and control epithelium in the non-surgical horns collected by laser micro dissection, obtained from Stat3 floxed/floxed mice as a control and uterine specific Stat3 conditional knockout mice in which uterine epithelial, stromal and myometrial regeneration were severely retarded.mouse uteri were transplanted into the artificial defects of recipient mouse uteri, and discovered that STAT3 accelerates uterine regeneration. In this project, we aims to identify more specific molecular regulation mechanism downstream of STAT3, comparing transcriptome between regenerating epithelium, cell-supplying epithelium in the surgical sites, and control epithelium in the non-surgical horns collected by laser micro dissection, which are obtained from uterine specific Stat3 conditional knockout mice in which uterine epithelial, stromal and myometrial regeneration were severely retarded and floxed mice as a control.