CRISPR-BASED GENOME MODIFICATION AND REGULATION
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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PROBLEM TO BE SOLVED: To provide methods of using modified RNA-guided endonucleases for expression in a eukaryotic cell or embryo and the RNA-guided endonucleases for targeted genome modification in the eukaryotic cell or embryo. SOLUTION: Provided is a method of introducing， into a eukaryotic cell， at least one RNA-guided endonuclease comprising at least one nuclear localization signal or a nucleic acid that encodes at least one RNA-guided endonuclease comprising at least one nuclear localization signal. The at least one RNA-guided endonuclease is a clustered regularly interspersed short palindromic repeats (CRISPR)/CRISPR-associated (Cas) (CRISPR/Cas) II system protein， which CRISPR/Cas II system protein is a Cas9 protein. SELECTED DRAWING: None
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
