Next Generation Sequencing analysis using splenic DCs freshly isolated from Wild Type and TBK1-DKO mice

In this experiment, we analyzed the gene expression profile of WT and TBK1-deficient splenic DCs by RNA sequencing based on three independent samples. The results revealed significant alterations in the expression of a number of genes, most notably the enhanced expression of a large subset of IFN-responsive genes in the TBK1-deficient DCs. Fresh splenic DC mRNA profiles of 8-week old wild type (WT) and TBK1-DKO mice were generated by deep sequencing, in triplicate本实验基于三份独立样本，通过RNA测序（RNA sequencing）分析了野生型（WT）与TBK1缺陷型脾脏树突状细胞的基因表达谱。结果显示，大量基因的表达发生显著改变，其中TBK1缺陷型树突状细胞内，干扰素应答基因（IFN-responsive genes）的庞大亚群表达上调尤为显著。本研究通过深度测序（deep sequencing）获取了8周龄野生型及TBK1双缺陷（TBK1-DKO）小鼠的新鲜脾脏树突状细胞mRNA表达谱，实验设置三次生物学重复。