Data for: Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum

Simultaneous, parental RNA interference (pRNAi) mediated knockdown of Hedgehog and Decapentaplegic (Dpp) signaling components, Pt-patched (Pt-ptc) and Pt-dpp, respectively, exhibited serious defects in the formation of the major embryonic axes in the model spider Parasteatoda tepidariorum. This paper describes a dataset of a custom oligonucleotide two-colour microarray analysis that was carried out to compare the transcript expression levels between untreated (normal) and Pt-ptc + Pt-dpp double pRNAi embryos at late stage 5. Array spots that showed the intensity ratio of [Pt-ptc + Pt-dpp double pRNAi]/[normal] <0.6 were categorized as positive. The expressions of most, not all, of the transcripts related to the positive array spots were examined in embryos by whole-mount in situ hybridization. Some of the stained embryos showed distinct patterns of gene expression. The data presented may be useful for characterizing the mechanisms of embyronic patterning in spider embryos.

本论文描述了一项针对模型蜘蛛帕拉斯特阿塔多德（Parasteatoda tepidariorum）胚胎中主要胚胎轴形成缺陷的研究。该研究通过同步、双亲性RNA干扰（pRNAi）手段，分别针对Hedgehog和Decapentaplegic（Dpp）信号通路组分进行敲低，即Pt-patched（Pt-ptc）和Pt-dpp。研究发现，上述处理在晚期5期胚胎中导致显著的胚胎轴形成缺陷。研究过程中，通过定制寡核苷酸双色微阵列分析，对比了未处理（正常）与Pt-ptc + Pt-dpp双pRNAi胚胎间的转录表达水平。微阵列斑点中，[Pt-ptc + Pt-dpp双pRNAi]/[正常]的强度比率低于0.6者被划分为阳性。大部分而非全部与阳性阵列斑点相关的转录本表达通过原位杂交在胚胎中进行检测。部分染色胚胎展示了基因表达的独特模式。所提供数据可能有助于阐释蜘蛛胚胎胚胎形成机理。